Project Description

Warning:  This is an archival page that does not reflect the current equipment on this microscope.  To see the current microscope configuration, click here.

Lucille

Yokogawa spinning disk confocal on an inverted Nikon Ti fluorescence microscope. Also configured for FRAP and widefield imaging.

Trained Users Only: Request FRAP alignment here prior to use

Imaging modalities

  • DIC
  • Phase contrast
  • Widefield fluorescence
  • Spinning disk confocal
  • Live cell imaging – short term
  • FRAP / Photoactivation

Hardware Components

  • Nikon Ti motorized inverted microscope with Perfect Focus System
  • Yokagawa CSU-X1 spinning disk confocal with Spectral Applied Research Aurora Borealis modification
  • Spectral Applied Research LMM-5 laser merge module with solid state lasers: 445nm (75mW), 488nm (100mW), 561nm (100mW) & 642nm (100mW. Laser line selection is controlled by AOTF.

  • Hamamatsu ORCA-R2 cooled CCD camera (confocal: 6.45 µm2 photodiode)

  • Hamamatsu ORCA-R2 cooled CCD camera (widefield: 6.45 µm2 photodiode)

  • Prior Proscan II motorized stage and shutters
  • Photonic Instruments MicroPoint laser targeting system for FRAP or photo-activation
  • Lumencor SOLA fluorescence light source
  • Okolab Stage Top Incubator for heating or cooling and CO2
  • MetaMorph image acquisition software
This instrument uses Class 3b lasers. All users must attend laser safety training.

Filter Spectra

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.

Illumination Setting Excitation Dichroic Emission
442 442nm 447/515/642 Triple 480/40
488 488nm 405/491/561/642 Quad 525/50
515 515nm 447/515/642 Triple 540/30
561 561nm 405/491/561/642 Quad 620/60
642 642nm 405/491/561/642 Quad 700/75
Widefield DAPI 395/25 400lp 460/25
Widefield FITC 480/40 505lp 535/50
Widefield TRITC 560/40 595lp 630/60