Project Description
Buster
Widefield inverted fluorescence microscope with incubation chamber.
Previous Configurations
Imaging modalities
- DIC
- Phase contrast
- Widefield fluorescence
- Live cell imaging – long term
Hardware Components
- Nikon Ti2 motorized inverted microscope with Perfect Focus System
- DIC, phase, and epi-fluorescence optics
Hamamatsu Flash4.0 sCMOS camera (6.5 µm2 photodiode)
- Nikon motorized stage and emission filter wheel
- Lumencor Spectra-X light engine for fluorescence illumination
- NIS Elements image acquisition software
- OkoLab 37° C, 5% CO2 cage microscope incubator
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Optical Config | Excitation | Emission |
|---|---|---|
| DAPI | 395/25 | 460/50 |
| GFP | 480/40 | 535/50 |
| RFP-mCherry | 560/40 | 630/75 |
| Quad-DAPI | 402/15 | 455/50 |
| Quad-FITC | 490/20 | 525/36 |
| Quad-TRITC | 555/25 | 605/52 |
| Quad-Cy5 | 645/30 | 705/72 |
| Quad 402-525 | 402/15 | 525/36 |
| Dual-CFP | 430/24 | 470/24 |
| Dual-YFP | 500/20 | 535/30 |
| Dual-FRET | 430/24 | 535/30 |