Project Description
Warning: This is an archival page that does not reflect the current equipment on this microscope. To see the current microscope configuration, click here.
Buster
Widefield inverted Nikon Ti fluorescence microscope with incubation chamber.
Imaging modalities
- DIC
- Phase contrast
- Widefield fluorescence
- Live cell imaging – long term
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
- DIC, phase, and epi-fluorescence optics
- Hamamatsu ORCA-R2 cooled CCD camera
- Nikon motorized stage and filter wheels
- Lumencor SOLA light engine for fluorescence illumination
- NIS Elements image acquisition software
- OkoLab 37° C, 5% CO2 cage microscope incubator
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Optical Config | Excitation | Emission |
|---|---|---|
| DAPI | 395/25 | 460/50 |
| GFP | 480/40 | 535/50 |
| RFP-mCherry | 560/40 | 630/75 |
| Quad-DAPI | 402/15 | 455/50 |
| Quad-FITC | 490/20 | 525/36 |
| Quad-TRITC | 555/25 | 605/52 |
| Quad-Cy5 | 645/30 | 705/72 |
| Quad 402-525 | 402/15 | 525/36 |
| Dual-CFP | 430/24 | 470/24 |
| Dual-YFP | 500/20 | 535/30 |
| Dual-FRET | 430/24 | 535/30 |