Project Description

Surely

Point scanning confocal with tunable detection on an inverted microscope.

Imaging modalities

  • Point-scanning confocal
  • DIC
  • Resonant Scanning
  • Live Imaging – Short or Long term
  • 32-channel Spectral Imaging
  • FRAP / Photoactivation
  • Image Montage

Hardware Components

  • Nikon Ti2-E motorized inverted microscope with Perfect Focus System

  • Nikon AX R scan head with galvo and resonant scanners

  • DUX-VB4 Detector (2 Tunable GaAsP, 1 Filter-Based GaAsP, and 1 Filter-Based High-Sensitivity PMT). Freely tunable emission bands with ± 1nm accuracy.

  • 8 Laser Lines: 405, 445, 488, 514, 561, 594, 640, and 730 nm

  • Prior Piezo Z stage (600 micron)

  • Tokai-Hit stage-top incubation system with 5% CO2

  • Lumencor SOLA fluorescence light source

  • Opti-Microscan XY Galvo Scanner (Frap, Photoactivation) with 405 and 473nm lasers
  • NIS Elements acquisition software
This instrument uses Class 3b lasers. All users must attend laser safety training.