Project Description
Warning: This is an archival page that does not reflect the current equipment on this microscope. To see the current microscope configuration, click here.
Lucille Two
Yokogawa spinning disk confocal on an inverted Nikon Ti fluorescence microscope.
Imaging modalities
- DIC
- Phase contrast
- Live cell imaging – short term
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
- Yokagawa CSU-X1 spinning disk confocal with Spectral Applied Research Aurora Borealis modification
- Spectral Applied Research LMM-5 laser merge module with solid state lasers: 445nm (75mW), 488nm (100mW), 515nm (100mW), 561nm (100mW) & 642nm (100mW) lasers. Laser line selection is controlled by AOTF.
Hamamatsu ORCA-R2 cooled CCD camera (6.45 µm2 photodiode)
- Prior Proscan III motorized stage and shutters
- Lumencor SOLA fluorescence light source
- Okolab Stage Top Incubator
- MetaMorph image acquisition software
This instrument uses Class 3b lasers. All users must attend laser safety training.
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Illumination Setting | Excitation | Dichroic | Emission |
|---|---|---|---|
| 442 | 442nm | 447/515/642 Triple | 480/40 |
| 488 | 488nm | 405/491/561/642 Quad | 525/50 |
| 515 | 515nm | 447/515/642 Triple | 535/30 |
| 515 (with EMCCD) | 515nm | 447/515/642 Triple | 540/30 |
| 561 | 561nm | 405/491/561/642 Quad | 620/60 |
| 642 | 642nm | 405/491/561/642 Quad | 700/75 |
| Widefield DAPI | 395/25 | 400lp | E420LP |
| Widefield FITC | 480/40 | 505lp | 535/50 |
| Widefield TRITC | 560/40 | 595lp | 645/75 |