Project Description
Tobias
Nikon 3D N-STORM Super-resolution / TIRF microscope
This microscope retired in April 2024, and is no longer available for use.
Imaging modalities
- DIC
- Phase contrast
- TIRF
- STORM super-resolution imaging
- 3D-STORM super-resolution imaging
Hardware Components
Nikon Ti motorized inverted microscope with Perfect Focus System
- Nikon TIRF illuminator
- Nikon 3D-STORM system
- Agilent MLC400B laser launch with 405nm (20mW), 488nm (50mW), 561nm (50mW) & 647nm (125mW) power (measured at fiber optic)
Andor DU-897 EM-CCD camera (16 µm2 photodiode)
Hamamatsu ImagEM (16 µm2 photodiode) with dualView
Prior Proscan II filter wheels and shutters
- Lumencor SOLA fluorescence light source
This instrument uses Class 3b lasers. All users must attend laser safety training.
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
Tobias has 4 laser lines: 405, 488, 561, and 647nm
| Optical Config | Excitation | Dichroic | Notch | Emission |
|---|---|---|---|---|
| TIRF 488 | ZET405/488/561/647x | ZT405/488/561/647rpc | ZET405/488/561/647m | ET 525/50 M |
| TIRF 561 | ZET405/488/561/647x | ZT405/488/561/647rpc | ZET405/488/561/647m | ET 600/50 M |
| TIRF 647 | ZET405/488/561/647x | ZT405/488/561/647rpc | ZET405/488/561/647m | ET 700/75 M |