Project Description
Warning: This is an archival page that does not reflect the current equipment on this microscope. To see the current microscope configuration, click here.
Michael
Widefield inverted Nikon Ti fluorescence microscope with incubation chamber.
Imaging modalities
- DIC
- Phase contrast
- Widefield fluorescence
- Live cell imaging – long term
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
- DIC, phase, and epi-fluorescence optics
- Hamamatsu ORCA-ER cooled CCD camera
- Prior Proscan III motorized stage and shutters
- Lumencor Spectra-X light engine for fluorescence illumination
- MetaMorph image acquisition software
- OkoLab 37° C, 5% CO2 cage microscope incubator
Filter Spectra
Filters from Chroma
| Illumination Setting | Excitation | Dichroic | Emission |
|---|---|---|---|
| Camera_405x515m | 395/25 | Lumencor Quad | 515/30 |
| Camera_GFP | 470/30 | Lumencor Quad | 515/30 |
| Camera_GFPexRFPem | 470/30 | Lumencor Quad | 632/60 |
| Camera_RFP | 575/22 | Lumencor Triple | 632/60 |
| Camera_RFPexCy5em | 575/22 | Lumencor Quad | 705/72 |
| Camera_Cy5 | 640/30 | Lumencor Quad | 705/72 |
| Camera_CFP | 440/20 | Lumencor Triple | 475/20 |
| Camera_YFP | 508/24 | Lumencor Triple | 540/21 |
| Camera_FRET | 440/20 | Lumencor Triple | 540/21 |
| Eye_GFP | 470/30 | B-2E/C | 535/40 |
| Eye_RFP | 575/22 | HcRED1 | 640/50 |
| Eye_YFP | 508/24 | HQ:Y GFP | 535/30 |