Project Description
Michael
Widefield inverted Nikon Ti2 fluorescence microscope optimized for slide-scanning.
Previous Configurations
Imaging modalities
- Brightfield
- Widefield fluorescence
Hardware Components
- Nikon Ti2 motorized inverted microscope with Perfect Focus System
- Brightfield and epi-fluorescence optics
Nikon DS-Qi2 large-format CMOS camera (7.3 µm2 photodiode)
Prior motorized xy-stage
- Lumencor Spectra-X light engine for fluorescence illumination
- NIS-Elements image acquisition software
Filter Spectra
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Illumination Setting | Excitation | Emission |
|---|---|---|
| Widefield Blue | 395/25 | 460/50 |
| Widefield Green | 466/40 | 525/50 |
| Widefield Red | 562/40 | 641/75 |
| Widefield Far-Red | 618/50 | 698/70 |