Project Description
Lucille
Yokogawa spinning disk confocal on an inverted Nikon Ti fluorescence microscope.
Previous Configurations
Imaging modalities
- DIC
- Phase contrast
- Widefield fluorescence
- Spinning disk confocal
- Live cell imaging – short term
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
Yokagawa CSU-X1 spinning disk confocal.
- Nikon LUN-F XL solid state laser combiner: 405 (80mW), 445 (35mW), 488 (80mW), 514 (50mW), 561 (65mW), 640nm (60mW)
Hamamatsu ORCA-Fusion BT sCMOS camera (confocal: 6.5 µm2 photodiode)
- Prior Proscan II motorized stage and shutters
- Lumencor SOLA fluorescence light source
- Okolab Stage Top Incubator for heating or cooling and CO2
NIS-Elements image acquisition software
This instrument uses Class 3b lasers. All users must attend laser safety training.
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Illumination Setting | Excitation | Dichroic | Emission |
|---|---|---|---|
| 442 | 442nm | 447/515/642 Triple | 480/40 |
| 488 | 488nm | 405/491/561/642 Quad | 525/50 |
| 515 | 515nm | 447/515/642 Triple | 540/30 |
| 561 | 561nm | 405/491/561/642 Quad | 620/60 |
| 642 | 642nm | 405/491/561/642 Quad | 700/75 |
| Widefield DAPI | 395/25 | 400lp | 460/25 |
| Widefield FITC | 480/40 | 505lp | 535/50 |
| Widefield TRITC | 560/40 | 595lp | 630/60 |