Project Description
Lucille Two
Yokogawa W1 spinning disk confocal on an inverted Nikon Ti fluorescence microscope. (*image not updated)
Previous Configurations
Imaging modalities
- DIC
- Phase contrast
- Live cell imaging – short term
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
Yokagawa CSU-W1 spinning disk confocal
Nikon LUN-F XL solid state laser combiner: 405 (80mW), 445 (35mW), 488 (80mW), 514 (50mW), 561 (65mW), 640nm (60mW)
Hamamatsu ORCA-Fusion BT CMOS camera (6.5 µm2 photodiode)
- Prior Proscan III motorized stage and shutters
- Lumencor SOLA fluorescence light source
- Okolab Stage Top Incubator
NIS-Elements image acquisition software
This instrument uses Class 3b lasers. All users must attend laser safety training.
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Illumination Setting | Excitation | Dichroic | Emission |
|---|---|---|---|
| 442 | 442nm | 447/515/642 Triple | 480/40 |
| 488 | 488nm | 405/491/561/642 Quad | 525/50 |
| 515 | 515nm | 447/515/642 Triple | 535/30 |
| 515 (with EMCCD) | 515nm | 447/515/642 Triple | 540/30 |
| 561 | 561nm | 405/491/561/642 Quad | 620/60 |
| 642 | 642nm | 405/491/561/642 Quad | 700/75 |
| Widefield DAPI | 395/25 | 400lp | E420LP |
| Widefield FITC | 480/40 | 505lp | 535/50 |
| Widefield TRITC | 560/40 | 595lp | 645/75 |