Project Description
Warning: This is an archival page that does not reflect the current equipment on this microscope. To see the current microscope configuration, click here.
George Michael
Inverted Nikon Ti fluorescence microscope optimized for fast acquisition and slide scanning with a large variety of filter configurations
Imaging modalities
- DIC
- Phase contrast
- Fluorescence
- Ratio imaging: Fura, FRET, biosensors
- Live cell imaging – short term
- Image montages for large areas
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
- Hamamatsu ORCA-R2 cooled CCD camera
- Prior Proscan III linear-encoded motorized stage, excitation and emission wheels and shutters
- Prior LumenPro fluorescence light source with excitation and attenuation wheels
- MetaMorph image acquisition software
- Tokai Hit stage top incubator (37˚C and 5% CO2)
Filter Spectra
Filters from Chroma
| Optical Config | Excitation | Dichroic | Emission |
|---|---|---|---|
| Filter DAPI | AT 350/50 | 400lp | ET 460/50 |
| Filter FITC | HQ 480/40 | 505lp | ET 535/50 |
| Filter TRITC | ET 545/30 | 570lp | ET 620/60 |
| Filter Cy5 | ET 620/60 | 660lp | ET 700/75 |
| Wheel UV | ET 402/15 | 69002bs | ET 460/40 |
| Wheel CFP | ET 430/24 | 69008bs | ET 470/24 |
| Wheel GFP | ET 490/20 | 69002bs | ET 535/50 |
| Wheel YFP | ET 500/20 | 69008bs | ET 535/30 |
| Wheel FRET | ET 430/24 | 69008bs | ET 535/30 |
| Wheel RFP | ET 572/35 | 69002bs | ET 632/60 |
| Fura 340 | ET 340x | 400lp | ET 510/80 |
| Fura 380 | ET 380x | 400lp | ET 510/80 |