Project Description
George Michael
Inverted Nikon Ti2 fluorescence microscope optimized for fast acquisition and short-term live imaging, with a large variety of filter configurations
Previous Configurations
Imaging modalities
- DIC
- Phase contrast
- Fluorescence
- Ratio imaging: FRET, biosensors
- Live cell imaging – short term
- Image montages for large areas
Hardware Components
- Nikon Ti2 motorized inverted microscope with Perfect Focus System
Hamamatsu ORCA-R2 cooled CCD camera (6.45 µm2 photodiode)
- Nikon motorized XY-stage
Hamamatsu Flash4.0 V2+ sCMOS camera (6.5 µm2 photodiode)
- Lumencor SpectraX fluorescence light source
- MetaMorph image acquisition software
- NIS-Elements Image Acquisition Software
- Tokai Hit stage top incubator (37˚C and 5% CO2)
Filter Spectra
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Optical Config | Excitation | Dichroic | Emission |
|---|---|---|---|
| Widefield Blue | 395/25 | T425lpxr | ET460/50m |
| Widefield Dual Green | 470/24 | 59022bs | BrightLine 525/50 |
| Widefield Dual Red | 575/25 | 59022bs | BrightLine 641/75 |
| Widefield Dual FRET | 470/24 | 59022bs | BrightLine 641/75 |
| Widefield Triple Cyan | 440/20 | 69008bs | ET470/24m |
| Widefield Triple Yellow | ET500/20x | 69008bs | ET535/30mm |
| Widefield Triple Red | 575/25 | 69008bs | BrightLine 641/75 |
| Widefield Triple FRET | 440/20 | 69008bs | ET535/30mm |
| Widefield Triple Red | 640/30 | T660lpxr | BrightLine 698/70 |