Project Description
George
Yokogawa spinning disk confocal on an inverted Nikon Ti fluorescence microscope with incubation enclosure.
Previous Configurations
Imaging modalities
- DIC
- Phase contrast
- Widefield fluorescence
- Spinning disk confocal
- Live cell imaging
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
Yokagawa CSU-X1 spinning disk confocal
Nikon LUN-F XL solid state laser combiner: 405 (80mW), 445 (35mW), 488 (80mW), 514 (50mW), 561 (65mW), 640nm (60mW)
Hamamatsu ORCA-Fusion BT sCMOS camera (6.5 µm2 photodiode)
- Prior Proscan II motorized stage and shutters
NIS Elements image acquisition software
- Lumencor SOLA fluorescence light source
- OkoLab 37°C, 5% CO2 cage microscope incubator
This instrument uses Class 3b lasers. All users must attend laser safety training.
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Illumination Setting | Excitation | Dichroic | Emission |
|---|---|---|---|
| 447 | 447nm | 447/515/642 Triple | 480/40 |
| 491 | 491nm | 405/491/561/642 Quad | 525/50 |
| 561 | 561nm | 405/491/561/642 Quad | 620/60 |
| 561 TMR | 561nm | 405/491/561/642 Quad | 605/70 |
| 642 | 642nm | 405/491/561/642 Quad | 700/75 |
| Widefield FITC | 480/40 | 505lp | 535/50 |
| Widefield TRITC | 540/25 | 595lp | 620/60 |
| Widefield CFP | 436/20 | 455lp | 480/40 |
| Widefield YFP | 500/20 | 515lp | 535/30 |