Project Description

Gene Parmesan

Widefield inverted fluorescence microscope with incubation chamber.

Imaging modalities

  • DIC
  • Phase contrast
  • Widefield fluorescence
  • Live cell imaging – long term

Hardware Components

  • Nikon Ti2 motorized inverted microscope with Perfect Focus System
  • DIC, phase, and epi-fluorescence optics

  • Hamamatsu Flash4.0 sCMOS camera (6.5 µm2 photodiode)

  • Nikon motorized stage and emission filter wheel

  • Lumencor Sola light engine for fluorescence illumination

  • NIS Elements image acquisition software

  • Tokai Hit 37° C, 5% CO2 cage microscope incubator

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