Project Description
Barry Zuckerkorn (Retired 9/2024)
Widefield inverted fluorescence microscope with monochrome and color cameras.
Imaging modalities
- Brightfield, including RGB color imaging
- Widefield fluorescence
Hardware Components
- Nikon Ti2 motorized inverted microscope with Perfect Focus System
- Brightfield and epi-fluorescence optics
Hamamatsu Flash4.0 LT camera (6.5 µm2 photodiode)
Nikon DS-Fi3 color camera
- Lumencor Sola light engine for fluorescence illumination
- Nikon white light LED for brightfield illumination
- Nikon motorized xy-stage
- NIS-Elements image acquisition software
Filter Spectra
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
| Illumination Setting | Excitation | Emission |
|---|---|---|
| Widefield Blue | 395/25 | 460/50 |
| Widefield Green | 466/40 | 525/50 |
| Widefield Red | 562/40 | 641/75 |
| Widefield Far-Red | 618/50 | 698/70 |