Project Description

Note:  This is an archival page.  Oscar was retired on 1/23/2018.  The replacement microscope (also usable for color transmitted light imaging) is Barry Zuckerkorn.

Oscar

Nikon 80i Upright Microscope

Imaging modalities

  • DIC
  • Phase contrast
  • Widefield fluorescence
  • Imaging stitching/montage

Hardware Components

  • Nikon 80i upright microscope
  • DIC, phase, and epi-fluorescence optics
  • Hamamatsu ORCA-100 cooled CCD camera
  • Nikon DS-Fi1 color camera
  • Lumencor SOLA light engine for fluorescence illumination
  • Prior Proscan III motorized stage and shutters
  • NIS Elements acquisition software
  • MetaMorph image acquisition software

Filter Spectra

Filters from Chroma

Compare the fluorescence spectra of your fluorophore to the filters on this microscope.