Project Description

Maeby

Nikon A1R point scanning confocal with spectral detection and resonant scanner, on an inverted Nikon Ti microscope.

Imaging modalities

  • Point-scanning confocal
  • DIC
  • Resonant Scanning
  • Live Imaging – Short or Long term
  • 32-channel Spectral Imaging
  • FRAP / Photoactivation
  • Interference Reflection Imaging
  • Image Montage

Hardware Components

  • Nikon Ti-E motorized inverted microscope with Perfect Focus System
  • Nikon A1R scan head with spectral detector and resonant scanners

  • 4 Laser Lines (405nm, 488nm, 561nm, and 640nm)

  • Tokai-Hit stage-top incubation system with 5% CO2
  • Lumencor SOLA fluorescence light source
  • NIS Elements acquisition software
This instrument uses Class 3b lasers. All users must attend laser safety training.

Filter Spectra

Filters from Chroma

Excitation Dichroic Emission
404nm 405/488/561/647 quad 450/50
488nm 405/488/561/647 quad 525/50
561nm 405/488/561/647 quad 595/50
647nm 405/488/561/647 quad 700/75