Project Description
Gob
Widefield inverted Nikon Ti fluorescence microscope with incubation chamber.
Imaging modalities
- DIC
- Phase contrast
- Widefield fluorescence
- Live cell imaging – long term
Hardware Components
- Nikon Ti motorized inverted microscope with Perfect Focus System
- DIC, phase, and epi-fluorescence optics
Hamamatsu ORCA-ER cooled CCD camera (6.45 µm2 photodiode)
- Prior Proscan III motorized stage and shutters
- Lumencor SOLA light engine for fluorescence illumination
- Prior LED light source for transmitted light
- MetaMorph image acquisition software
- OkoLab 37°C, 5% CO2 cage microscope incubator
Filter Spectra
Filters from Chroma
Compare the fluorescence spectra of your fluorophore to the filters on this microscope.
Widefield Triple Cyan440/20475/20Widefield Triple FRET440/20540/21Widefield Triple Red575/22632/60
| Optical Config | Excitation | Emission |
|---|---|---|
| CFP | 436/20 | 480/40 |
| GFP | 480/40 | 535/50 |
| YFP | 500/20 | 535/30 |
| RFP | 560/40 | 630/75 |
| Cy5 | 620/60 | 700/75 |
| Widefield Triple Yellow | 508/24 | 540/21 |